Nippon Genetics FastGene Scriptase II - 20000 Units at 200U/uL; 100 Reactions

Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV's reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.

• Applications: quantification of gene expression, RT-qPCR, next generation sequencing, low RNA concentration, difficult templates
• Lower RNase H activity for longer cDNA: The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12kBp.
• Engineered enzymes – optimised for qPCR: The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS, the resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants